Journal: PloS one

Article Title: Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy.

doi: 10.1371/journal.pone.0066759

Figure Lengend Snippet: Figure 1. Gas6 expression in human IgA nephropathy. Biopsy samples were immunostained using indirect immunohistochemistry procedure with anti-Gas6 antibody. Representative images are shown in (A) from control patients, (B) from IgA nephropathy cases immunostained in epithelial cells, and (C) from IgA nephropathy cases immunostained in endothelial and mesangial cells. The original magnification was X200. Endo/mes, Endothelial and mesangial. doi:10.1371/journal.pone.0066759.g001

Article Snippet: Three times diluted samples were then applied on the ELIZA plate (human Gas6 Duoset ELIZA Development kit, R&D Systems).

Techniques: Expressing, Immunohistochemistry, Control

Journal: PloS one

Article Title: Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy.

doi: 10.1371/journal.pone.0066759

Figure Lengend Snippet: Figure 2. Gas6 stained area in human IgA nephropathy. Gas6 stained area is expressed as a percentage of total glomerular area occupied by Gas6 immunostained area. For each sample, at least eight glomerular profiles per patient were measured. IgA epi, IgA epithelial type. doi:10.1371/journal.pone.0066759.g002

Article Snippet: Three times diluted samples were then applied on the ELIZA plate (human Gas6 Duoset ELIZA Development kit, R&D Systems).

Techniques: Staining

Journal: PloS one

Article Title: Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy.

doi: 10.1371/journal.pone.0066759

Figure Lengend Snippet: Figure 3. Correlation of Gas6 stained area with prognostic factors. Gas6 stained area in human IgA nephropathy correlated with (A) Oxford mesangial hypercellularity score and (B) urine protein excretion. N = 28. Oxford M score, Oxford mesangial hypercellularity score. doi:10.1371/journal.pone.0066759.g003

Article Snippet: Three times diluted samples were then applied on the ELIZA plate (human Gas6 Duoset ELIZA Development kit, R&D Systems).

Techniques: Staining

Journal: PloS one

Article Title: Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy.

doi: 10.1371/journal.pone.0066759

Figure Lengend Snippet: Figure 4. Inverse correlation of Gas6 stained area with p27 positive cell number. (A) Biopsy samples were immunostained using indirect immunohistochemistry procedure with anti-p27 antibody. p27 positive cell number was counted. The result was divided by glomeruli number. For each sample, at least eight glomerular profiles per patient were measured. p27 positive cell number correlated with Oxford mesangial hypercellularity score. (B) Gas6 stained area inversely correlated with p27 positive cell number. N = 28. Oxford M score, Oxford mesangial hypercellularity score. doi:10.1371/journal.pone.0066759.g004

Article Snippet: Three times diluted samples were then applied on the ELIZA plate (human Gas6 Duoset ELIZA Development kit, R&D Systems).

Techniques: Staining, Immunohistochemistry

Journal: PloS one

Article Title: Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy.

doi: 10.1371/journal.pone.0066759

Figure Lengend Snippet: Figure 6. Double immunohistochemistry of Dtk and Axl with cell specific markers or Gas6. Biopsy samples were immunostained using indirect immunohistochemistry procedure with (A) anti-Dtk, anti-Nephrin (a podocyte marker), (B) anti-Dtk, anti-Gas6 or (C) anti-Axl, anti-CD34 (an endothelial cell marker), (D) anti-Axl, anti-Gas6 antibody. (A,B) Dtk immunostaining was detected in both control and IgA nephropathy, mostly merged with Nephrin. It was also merged with Gas6 in epithelial type IgA nephropathy. (C,D) Axl immunostaining was detected in both control and IgA nephropathy, mostly merged with CD34. It was also merged with Gas6 in endothelial and mesangial type IgA nephropathy. X200. IgAN, IgA nephropathy. IgA epi, IgA epithelial type. IgA endo/mes, IgA endothelial and mesangial type. doi:10.1371/journal.pone.0066759.g006

Article Snippet: Three times diluted samples were then applied on the ELIZA plate (human Gas6 Duoset ELIZA Development kit, R&D Systems).

Techniques: Immunohistochemistry, Marker, Immunostaining, Control

Journal: PloS one

Article Title: Dual involvement of growth arrest-specific gene 6 in the early phase of human IgA nephropathy.

doi: 10.1371/journal.pone.0066759

Figure Lengend Snippet: Figure 7. Effect of Gas6 on Dtk and p27 expression in podocytes. Podocytes were differentiated and stimulated with recombinant Gas6 or TGFb1 for 24 hours. Cell lysates were subjected to immunoblotting with the antibodies indicated. Then, podocytes expressed Dtk. Gas6 could increase the expression of Dtk and reduce p27, as well as TGFb1. Representative data were shown from four independent experiments. Quantitative examinations of the band density for (B) Dtk and (C) p27 were shown. The columns and error bars are the mean 6 SD of four independent experiments. doi:10.1371/journal.pone.0066759.g007

Article Snippet: Three times diluted samples were then applied on the ELIZA plate (human Gas6 Duoset ELIZA Development kit, R&D Systems).

Techniques: Expressing, Recombinant, Western Blot